Trypan Blue for Cell Viability Staining

McMaster University
MIRC
Postdoctoral Researcher

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Company:

VWR

Product Name:

Trypan blue 0.4% in aqueous solution Ready-to-Use, sterile

Catalog Number:

72-57-1

We use this reagent for routine cell culture to determine the viability of our cells. The reagent works well as it is readily taken up by dead cells but excluded from live cells.

Experimental Design and Results Summary

Application

Cell culture

Starting Material

Mammalian cells

Tips

Make sure to store it in a dark place.

Results Summary

This dye works well to stain dead cells blue, leaving live cells translucent. It makes determining the viability of the culture very easy.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Good staining.

The Bad

Can form precipitate if left exposed to light.

The Bottom Line

Good dye to use in any routine cell culture.

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