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PE/Dazzle™ 594 anti-mouse CD48 Antibody
103437
HM48-1
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of B cells and antibody-secreting cells (ASC) in health and disease, in this experiment, we are looking for markers of PC.
Flow Cytometry
WT mouse splenocytes
Surface staining with CD48 PE-Dazzle (1/200), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
In this work, we were looking for surface markers that are differentially expressed by B cells (CD19+) and plasma cells (PC). CD48 is expressed by B cells. Here, we used CD19+ B cells to titrate the antibody.
It works well.
Antibody with good signal.