Clear Detection of Bands

MD Anderson Cancer Center - University of Texas
Cancer System Imaging (Diagnostic Imaging Division)
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Bio-Rad

Product Name:

Precision Plus Protein Dual Color Standards, 500 µl

Catalog Number:

1610374

I used the Precision Plus Protein Dual Color Standards for the molecular weight estimation on SDS-PAGE gels and western blots. The product runs well also at higher voltage and always gives me a clear detection of the bands also at lower KDa.

Experimental Design and Results Summary

Application

SDS page and Western blots

Starting Material

Protein sample

Tips

Dilute with sterile water to reach the same volume of the sample loaded for a more precise run and detection of the molecular weights (also good for imaging).

Results Summary

Precision Plus Protein Dual Color Standards very often. Last experiment I did was a SDS page to detect the molecular weight of a conjugate (antibody plus a drug) and the pure antibody. I obtained clear and smooth bands that lead me to estimate the molecular weights of my proteins and distinguish between the two samples.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Clear bands, easy molecular weights estimation.

The Bad

N/A

The Bottom Line

Precision Plus Protein Dual Color Standards is always give me the best bands to detect and estimate the molecular weights of my samples when doing SDS-page or Western blots.

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