RayBiotech
Human TNF-a Elisa
ELH-TNFa-1
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This kit was employed for the assessment of THP-1 inhibitory assays using MSCs cultured on different surfaces. Results were quite impressive by following trends according to the number of cells cultured, showing both high sensitivity and reproducibility.
THP-1 inhibitory assay
LPS treated THP-1 and Bone marrow derived MSCs
Be sure of using the appropriate diluent according to the nature of your sample.
I was able to appreciate hiw MSCs culture conditions can affect their immunomodulatory capacity by interfering on TNF-a secretion by LPS- treated THP-1 cells.
N/A
Easy to use.
Too expensive.
Overal it is a great product, with a well detailed protocol.