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ELISA MAX Deluxe set Human IL-6
430504
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Primary blood monocyte derived macrophages as a in vitro model. Collected conditioned media after treatments and measured IL6 levels
IL6 by ELISA
Conditioned media
Treated for 4h
ELISA is sensitive enough to detect at 1-5 pg level in the conditioned media. Took about 15 min to develop sufficient signal for reading. No background signal in the blank wells.
N/A
Easy to perform and needs about 5h to complete.
Do not provide wash buffer and stop solution.
Very good Kit for IL6 ELISA cell culture conditioned media.