Reasonably Good Antibody to Detect RFP-Tag

Pharmacology
PennState College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Rockland

Product Name:

Anti-RFP Antibody

Catalog Number:

600-901-379S

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: RFP Antibodies

We have been using this antibody to detect RFP tags in recombinant proteins. The antibody is specific and works well at the suggested concentration.

Experimental Design and Results Summary

Applications

Western Blot

Sample

E. coli expressed recombinant His-tagged protein

Primary Incubation

1:1000, overnight incubation at 4 degree Celsius

Blocking Agent

5% BSA in TBST

Secondary Incubation

Goat anti-chicken HRP Ab, dilution (1:2500) in 5% BSA , 1hr at RT

Tertiary Incubation

N/A

Detection

ECL

Results Summary

A good antibody with a very clear and strong signal. Highly recommended for the detection of RFP-tagged recombinant proteins.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Price and quality of results.

The Bad

None

The Bottom Line

A great antibody, less expensive, works well.

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