Good Antibody to Detect MAP2-Neuronal Marker

Pharmacology
PennState College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-MAP2 antibody

Catalog Number:

ab32454

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: MAP2 Antibodies

We used this antibody to detect MAP2 expression in mammalian cells. The antibody works well at the suggested dilution with some cross reactivity.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mammalian cell line whole cell lysate

Primary Incubation

1:1000, overnight incubation at 4 degree Celsius

Blocking Agent

5% BSA in TBST

Secondary Incubation

Goat anti-rabbit IgG HRP Ab, dilution (1:2500) in 5% BSA , 1hr at RT

Tertiary Incubation

N/A

Detection

ECL

Results Summary

Antibody reacted well to the MAP2 proteins from the whole cell lysate of the differentiated neuron. Overall it's a good antibody to detect the expression of MAP2. I recommend it.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Works well.

The Bad

Minor cross reactivity.

The Bottom Line

Works well and can be used to detect MAP2.

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