Easy to Use ELISA Kit, Suitable for Multiple Cells

Weill Cornell Medicine
Postdoctoral Researcher

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Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit

Catalog Number:

DCX130

Clone Number:

N/A

This kit was used to determine the concentration of this protein-of-interest in multiple cancer cell lines and in different conditions. Just normalize the cell numbers and get the supernatant, then follow the instruction of this kit.

Experimental Design and Results Summary

Applications

ELISA

Sample

Human PC3 cell lines

Primary Incubation

Room temperature, 2 hour

Blocking Agent

Blocking Agent provided in this kit

Secondary Incubation

Room temperature, 2 hour

Tertiary Incubation

Room temperature, 30 min

Detection

ELISA plate reader (96 well)

Results Summary

Upon detection, certain conditions will increase the secretion of this protein, which is consist with our expectation.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Reliable and good standard curve.

The Bad

Limit to ~72 wells available, besides the standards.

The Bottom Line

Expensive but reliable and easy to use.~8hour from start to end.

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