Effect of Chemoattractant on AKAP7 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Proteintech Group, Inc.

Product Name:

AKAP7 Polyclonal antibody

Catalog Number:

12591-1-AP

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: AKAP7 Antibodies

AKAP7 have been shown to regulate neuronal ion channels. AKAP7alpha is the first AKAP7 splice variant identified and it is a membrane bound protein containing a lipid modification domain in the amino terminus. It also shown to be interact with sodium channels in the brain, and is required for feedback inhibition of the epithelial Na+ channel. Here we checked the time course effect of chemoattractant by AKAP7 expression by chemoattractant.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

Mouse Lysate

Primary Incubation

1:1000 prepared in 1x TBST

Blocking Agent

5% Skim milk

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We did immunoprecipitation first and performed western blot to detect and analyzed the AKAP7 expression. We found that there is a time dependent increase of AKAP7 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

This is a antibody and works great for western blot.

The Bad

Price of this antibody is little expensive.

The Bottom Line

This antibody works great for both immunoprecipitation and western blot. Signal is great. Highly recommended.

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