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Alexa Fluor® 488 anti-Neurofilament H & M (NF-H/NF-M), Phosphorylated Antibody
837712
SMI 310
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Fixed (4% PFA) and cryoprotected (sucrose 10-30%) optic nerves were permeabilized with PBS-Triton 0.25 % for 20 min and blocked with PBS - 3% BSA – 0.1% Triton for 2h at RT. Optic nerve sections were incubated with 2.5 ug/mL of the Alexa Fluor® 488 anti-Neurofilament H & M (clone SMI 310) overnight at 4°C. Nuclei were counterstained with DAPI, and the slides were mounted with Dako mounting medium. The image was captured with a 40X objective with a Carl Zeiss DMi8 microscope.
Immunofluorescence
Optic nerve
2.5 ug/mL, overnight, 4°C
3% BSA – 0.1% Triton
N/A
Microscopy
Alexa Fluor® 488 anti-Neurofilament H & M (NF-H/NF-M), Phosphorylated Antibody was used to detect axons in mouse optic nerve samples. Antibody works perfectly at the defined conditions.
High quality, easy to use and good signal.
Needs a concentrated dilution, expensive.
Great antibody, highly recommended, would buy again.