Good 4E-BP1 Antibody for Western Blot

May 24, 2022

NanoEngineering
UC San Diego NanoEngineering Department
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling

Product Name:

4E-BP1 Antibody

Catalog Number:

9452

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: 4EBP1 Antibodies

I stimulated epithelial cells for 24 hours with TGF-b and I transfected eIF4 siRNA for 24 hours with starvation medium (0.5% FBS). I diluted the antibody 1:1000 in 5% BSA in TBST. Secondary antibody was diluted to 1:10000.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Epithelial cells

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

I stimulated epithelial cells for 24 hours with TGF-b and I transfected eIF4 siRNA for 24 hours with starvation medium (0.5% FBS). I diluted the antibody 1:1000 in 5% BSA in TBST. Secondary antibody was diluted to 1:10000.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

Antibody has great signal, highly recommended.

Share your experience with other scientists. Write a Review! »

Join the discussion