Cell Signaling
SirT3 (D22A3) Rabbit mAb
5490S
N/A
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I seed epithelial cells (lung cancer cells) for 24 hours and I treated with eIF4E siRNA for 24 hours. I evaluated SirT3 expression with western blot diluting it up to 1:1000 and incubating the membrane overnight at 4 °C.
Western Blot
Epithelial cells
1:1000, 4 °C, overnight
Milk 5% TBST
1:5000
Chemiluminescence
I was able to get some results
Band isn't so clear
Expensive but great, would buy again