Good Secondary Antibody for Western Blot

May 20, 2022

NanoEngineering
UC San Diego NanoEngineering Department
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

Anti-rabbit IgG, HRP-linked Antibody

Catalog Number:

7074

Clone Number:

N/A

I used this antibody several times and I always got great results. I diluted 1:10000 with 5% BSA in TBST and I incubated my membranes from 1 to 4 hours depending on the primary antibody at RT.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Endothelial cells

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

BSA 5% TBST

Secondary Incubation

1:10000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

I used this antibody several times and I always got great results. I diluted 1:10000 with 5% BSA in TBST and I incubated my membranes from 1 to 4 hours depending on the primary antibody at RT.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

Really recommended.

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