Beckman Coulter
CD4-APC-A700, 13B8.2, 0.5 mL, ASR
B10824
13B8.2
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Our lab continues to study human lymphocyte populations, including CD4+ and CD8+ T cells. In a previous review, I showed how CD4 FITC worked but was quite dim. Here, I review a CD4 conjugated to APC-A700 which produces a much brighter signal.
Flow Cytometry
Human WBCs
10 minutes at room temp
10% bovine serum
N/A
Navios Cytometer (equipped with red laser)
The results show a bright, excellent signal on the helper T cells with superb separation of the positives (purple) from the negatives (grey). The median fluorescence intensity is shown underneath the plot.
Strong, bright signal.
Lots of spillover issues.
This is a good antibody that yields a strong signal, but will create compensation issues in nearby channels (APC and APC-A750).