Great Integrin Beta 2 Antibody for Western Blot

May 18, 2022

Nanomedicine
Lazar Research Laboratories
Clinical Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Mouse/Canine Integrin beta 2/CD18 Antibody

Catalog Number:

AF2618

Clone Number:

N/A

I used this antibody to evaluate the expression of CD18 in inflammation sites. This antibody worked very well, resulting in a clear band and strong signal.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Murine cells line

Primary Incubation

1:1000, 4 °C, overnight

Blocking Agent

Milk 5% TBST

Secondary Incubation

1:2000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

Cd18 was detected very well. I wanted to evaluate CD18 for his ability to bind to the associated receptors on the inflamed endothelia.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Band is clear.

The Bad

N/A

The Bottom Line

Antibody has great signal, highly recommended.

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