Time Saver Antibody from Sino Biological

May 11, 2022

Pharmacology
PennState College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sino Biological

Product Name:

SARS-CoV / SARS-CoV-2 Spike antibody (HRP), Chimeric MAb

Catalog Number:

40150-D001-H

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: HRP Antibodies

Strong signal and very specific. Since HRP is conjugated, a secondary antibody is not required. Ideal for ELISA assays.

Experimental Design and Results Summary

Applications

ELISA

Sample

Recombinant SARS-CoV Spike RBD Protein

Primary Incubation

1:2500 30 min at room temperature

Blocking Agent

1% BSA

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

TMB solution used as substrate. OD at 450 nm after stopping the reaction.

Results Summary

We commonly use this HRP-conjugated antibody for detecting spike RBD detection using ELISA. We found the best results at 1:2500 dilution. With this, TMB solution works really well.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Very strong signal, specific, time saver and repeatable.

The Bad

N/A

The Bottom Line

Quality HRP-conjugated primary antibody for ELISA applications. Recommended.

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