Excellent Antibody for Detecting Spliced Isoform of XBP1

School of Natural Science
NUIG
Research Scientist

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Company:

BioLegend

Product Name:

Purified anti-XBP-1s Antibody

Catalog Number:

647501

Clone Number:

143F

Info:Supplier Page View Product Specs

Browse Similar Products: XBP1 Antibodies

This antibody is one of the best commercially available. It's specific for the isoform spliced of XBP1 (XBP1s). However, who uses this antibody should be aware of an unspecific band in close proximity (below) of the XBP1s band.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cell line of breast cancer, MDA-MB-231

Primary Incubation

1:1000. Overnight. Cold room.

Blocking Agent

5% Milk in PBS

Secondary Incubation

1:4000. 1 hour. Room temperature

Tertiary Incubation

N/A

Detection

Bioluminescence (HRP-conjugated)

Results Summary

This antibody is specific for XBP1. A gene knockout for XBP1 in MDA-MB-231 was able deplete the expression and its detection in WB. However, an unspecific band is detected in proximity of XBP1s. A 8% gel was used to properly separate the two bands.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specific antibody at a very good price.

The Bad

It always gives an unspecific band which might be confused by XBP1s.

The Bottom Line

Excellent antibody!

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