PNGase-F Used to Detect NTCP

University of Pittsburgh
Surgery
Research Scientist

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Company:

New England BioLabs

Product Name:

Remove-iT PNGase-F

Catalog Number:

P0706S

Used to detect the expression of NTCP by removing the N-linked sugar chains.

Experimental Design and Results Summary

Application

Western blot

Starting Material

Whole cell lysates

Tips

SDS could not be present in the lysis buffer since it inactivates the enzyme.

Results Summary

NTCP was detected by removing the N-linked sugar chains.

DOI or PMID #

N/A

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Summary

The Good

It works well.

The Bad

N/A

The Bottom Line

It works.

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