Dependable Anti-Neurofilament-M Antibody for Immunofluorescence

January 12, 2022

Angioscience
ECAS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermofisher

Product Name:

Anti-Neurofilament-M Antibody for Immunofluorescence

Catalog Number:

13-0700

Clone Number:

RMO-270

Really good antibody to label axons (neurofilament-M staining).

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Limb of mouse embryo.

Primary Incubation

1:300, overweekend incubation at 4C in 5%Normal donkey serum in 0,3% triton-x100 in PBS

Blocking Agent

5%Normal donkey serum in 0,3% triton-x100 in PBS

Secondary Incubation

Overnight at RT in 5%Normal donkey serum in 0,3% triton-x100 in PBS

Tertiary Incubation

None.

Detection

Confocal microscope.

Results Summary

Good staining of axons in the mouse embryo limb.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Quality.

The Bad

None.

The Bottom Line

I totally recommend this antibody.

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