Beckman Coulter
CD33-PC5.5, D3HL60.251, 0.5 mL, ASR
A70198
D3HL60.251
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CD33 is a versatile marker. Not many appreciate how useful it can be in identifying different blood cell subsets. In this review, my lab used CD33 to identify monocyte, granulocytes and even basophils.
Flow Cytometry
Peripheral blood or bone marrow
15 minutes - Room temperature
None
N/A
Navios flow cytometer (~700nm)
The staining is straightforward. The results are impressive. Shown in the image attached, a simple 2D dotplot with CD33 on the x-axis and side scatter on the y-axis. Staining with CD33 allowed the identification of monocytes (bright positive - in green), granulocytes (dim positive - in blue), lymphocytes (negative - in red), and even basophils (intermediate positive/low side scatter - in purple).
Easy prep and optimization. ASR quality.
Over-priced.
This antibody is very good and very useful for labs performing human WBC analysis. I recommend it!