Reliable NCS-1 Antibody

Neuroscience
UAB
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Anti-NCS-1 Antibody

Catalog Number:

sc-376206

Clone Number:

G-4

Info: Supplier Page View Product Specs

Browse Similar Products: NCS-1 Antibodies

I have used this antibody to detect Ncs-1 by western blot in a number of sample types. It has worked very well in all tested samples, from mouse brain to culture cells (PC12 and HEK293 cells). In all cases reactivity was quite good at 1:2,000 dilution. Band size was the same as predicted.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse brain

Primary Incubation

Incubation was done with Ab diluted to 1:2000 for 16h at 4ºC

Blocking Agent

BSA 4% in PBS

Secondary Incubation

Incubation was done with 2nd Ab diluted to 1:10,000 for 1h at room temperature

Tertiary Incubation

None

Detection

ECL

Results Summary

This antibody worked very well.

DOI or PMID #

32165725

Additional Notes

Related Categories

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Summary

The Good

reliable and sensible

The Bad

none

The Bottom Line

Great antibody

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