Donkey anti-Goat IgG Secondary Antibody is Great for IHC

Radiation Oncology
UAB
Research Scientist
November 26, 2021

Radiation Oncology
UAB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

Donkey anti-Goat IgG (H+L) Secondary Antibody, HRP

Catalog Number:

A15999

Clone Number:

N/A

Info: Supplier Page View Product Specs

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This antibody can be used for detecting goat antigens in tissue specimens for immunofluorescence/immunohistochemistry

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Human cervical cancer cells

Primary Incubation

1:200 overnight at 4 degrees

Blocking Agent

5% Donkey serum

Secondary Incubation

1:1000 at room temperature for 1 hour

Tertiary Incubation

10 minutes with TSA

Detection

Microscopy

Results Summary

FITC signaling was readily detectable in IHC using TSA (tyramide-based) kit for fluorescence

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very easy to use

The Bad

N/A

The Bottom Line

Highly reliable

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