Gentle Enzyme Solution for Trypsinization of Adherent Cells

November 22, 2021

University of Tennessee HSC
College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

ThermoFisher Scientific

Product Name:

TrypLE™ Express Enzyme (1X), no phenol red

Catalog Number:

12604013

I have used this TrypLE enzyme solution for the trypsinization of stem cells. It is an animal origin free and gentle to cells which makes it preferable for stem cells research.

Experimental Design and Results Summary

Application

Trypsinization of adherent cells.

Starting Material

Adherent human or mouse cells.

Tips

Standardize the time for your cells for good results.

Results Summary

Within 3-5 minutes, more than 90% of cells were detached from the surface of the flask.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Mild/gentle to cells results in good cell viability.

The Bad

Bit costly compared to trypsin-EDTA solution.

The Bottom Line

It is better to include in your research if you are dealing with sensitive cells and are concerned about their differentiation or viability during different passages.

Join the discussion