Mouse CD62L PE-Cy7 Works Great

Surgery
UCSF
Graduate Student
November 23, 2021

Surgery
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher

Product Name:

CD62L (L-Selectin) Monoclonal Antibody (MEL-14), PE-Cyanine7

Catalog Number:

25-0621-82

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: CD62L Antibodies

Staining splenic T cells to identify memory vs naive T cells based on CD44 and CD62L expression

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 dilution for 30 minutes at 4 degrees C

Blocking Agent

2.4G2 Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

LSRII FACS Analyzer

Results Summary

This antibody helped me classify most T cells in the as naive based on CD62L expression.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Clean and strong signal

The Bad

Could be cheaper

The Bottom Line

Works great for T cell staining

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