Abcam
Anti-Xanthine Oxidase [EPR4605] Antibody
ab109235
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SDS-PAGE resolved human protein extracts from cell line exposed to different time periods of a growth factor were immunoblotted and probed with the anti-Xanthine Oxidase antibody. The aim was to test the effect of time-course effects of growth factor on Xanthine Oxidase protein levels. The selection of antibody from Abcam was based on the information provided in the supplier antibody flyer.
Western Blot
Human cell protein lysate
Dilution (1:1000) in 5% BSA prepared in 1x TBST, overnight incubation at 4 degree Celsius
5% skimmed milk prepared in 1x TBST
Goat anti-rabbit IgG HRP Ab, dilution, dilution (1:4000) in 5% skimmed milk prepared in 1x TBST, 1hr at RT
None
ECL detection in X-Ray film
In the present study, an antibody from Abcam was used, which showed an excellent specificity towards Xanthine Oxidase. No non-specificity was detected.
Detected highly specific band of Xanthine Oxidase
Excellent antibody for the detection of Xanthine Oxidase