Basophil Depletion is Not the Best

Immunology
Immunology
Principal Investigator
October 19, 2021

Immunology
Immunology
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Ultra-LEAFTM Purified anti-mouse CD200R3

Catalog Number:

142216

Clone Number:

Ba13

Info: Supplier Page View Product Specs

Browse Similar Products: CD200R3 Antibodies

I used this clone for basophil depletion in mice and only received 50% efficacy. The vendor told me they never tested this clone in depletion and can't guarantee the performance.

Experimental Design and Results Summary

Applications

Block/Neutralize/Inhibit

Sample

Basophils

Primary Incubation

100 ug per mouse

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Cytometry

Results Summary

After antibody treatment, there are still 2% basophils remaining

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

It depleted some cells

The Bad

Not effective for in vivo study

The Bottom Line

Usable

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