eBiosciences
Podoplanin
53-5381-80
eBio8.1.1
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We study how tissue resident immune cells regulate tissue homeostasis. For this, it is important to visualise the immune cells of interest within their native tissues. Yet at first, we validate various antibodies for IF, using specific tissues as positive control(s).
Immunofluorescence
PLP fixed mouse (C57BL/6) kdney cryosections
1 in 100 overnight at room temperature
PBS with 1% BSA, 0.3% Triton X, 2% mouse serum, 2% rat serum
N/A
Zeiss 780 confocal microscope
18 um kidney cryosections were stained for CD31 (red), Podoplanin (green), and counterstained with DAPI (blue). Mice positive staining of podocytes (as expected).
Reliable staining
None
Reproducible staining of podoplanin.