Biolegend
ICAM1
116111
YN1/1.7.4
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We seek to unravel how tissue resident immune cells mediate tissue homeostasis. Hence, It is important to visualise the immune cells of interest within their native tissues. Yet at first, we validate various antibodies for IF, using specific tissues as positive control(s).
Immunofluorescence
PLP fixed mouse (C57BL/6) small intestine cryosections
1:100 overnigth at room temperature
PBS with 1% BSA, 0.3% Triton X, 2% mouse serum, 2% rat serum
N/A
Zeiss 780 confocal microscope
16 um cryosections of small intestine were stained for CD31 (red), ICAM1 (green), and counterstained with DAPI (blue)
Good, reliable staining
None
Good, reproducible staining of ICAM1 in cryosections.