Test Specificity of HLA-E

Protein Therapeutics
Biopharma
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen (Thermo)

Product Name:

HLA-E mono Antibody (3D12HLA-E)

Catalog Number:

14-9953-82

Clone Number:

3D12HLA-E

Info: Supplier Page View Product Specs

Browse Similar Products: HLA-E Antibodies

Looked at different clones of Anti-HLA-E for specificity to HLA-E MHC compared to HLA-A. 3D12HLA-E had good binding to HLA-E MHC and no interaction with HLA-A construct. Signal was strong for this antibody

Experimental Design and Results Summary

Applications

Protein: Protein interaction

Sample

Human HLA-E

Primary Incubation

5min

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Octet detection

Results Summary

Checked different clones of Anti-HLA-E. 3D12HLA-E had the highest signal to HLA-E MHC protein. This antibody also showed specificity to HLA-E over HLA-A. Binding was very tight.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good specificity and signal

The Bad

N/A

The Bottom Line

This antibody was specific to HLA-E vs other MHC proteins. The binding signal is very tight. This was a decent reagent to verify binding

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