Sensitive Phospho-PRAS40 Antibody

September 23, 2021

BMS
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Rabbit monoclonal anti-p-PRAS40 (Thr246) (C77D7)

Catalog Number:

2997

Clone Number:

N/A

Investigating insulin-dependent and insulin-independent signaling pathways under the context of obesity.

Experimental Design and Results Summary

Applications

Western Blot

Sample

F442A fibroblast cell cline

Primary Incubation

1:1000 4C overnight incubation in TBST buffer

Blocking Agent

5% non-fat milk

Secondary Incubation

anti-rabbit HRP antibody

Tertiary Incubation

NA

Detection

HRP substrate and x-ray film exposure

Results Summary

Good signal across all samples.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good signal

The Bad

Expensive

The Bottom Line

Antibody has great signal, highly recommended

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