Sensitive Antibody for Western Blot

September 17, 2021

BMS
UCSF
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Rabbit monoclonal anti-Akt (pan) (C67E7)

Catalog Number:

4691;

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: Anti-AKT Antibodies

Investigating insulin-dependent and insulin-independent signaling pathways under the context of obesity.

Experimental Design and Results Summary

Applications

Western Blot

Sample

human SGBS cells

Primary Incubation

1:1000 4C overnight incubation in TBST buffer

Blocking Agent

4%BSA

Secondary Incubation

anti-rabbit HRP antibody

Tertiary Incubation

1:10000

Detection

HRP substrate and x-ray film exposure

Results Summary

Good signal of total AKT level across all samples. Used as a control.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Good signal

The Bad

Expensive

The Bottom Line

Antibody has great signal, highly recommended.

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