Biolegend
PerCP/Cyanine5.5 anti-mouse CD45.1 Antibody
110728
A20
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at testing if we can detect CD45.1 in lymphocytes of the CD45.1 mouse, from which we will transfer cells into a recipient CD45.2 (B6).
Flow Cytometry
Mouse spleen CD45.1 and CD45.2 (B6)
surface staining with CD45.1 Percp-Cy5.5, 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
In the figure, we can observe staining of lymphocytes with CD45.1 on the right, while in a normal B6, which is CD45.2 there is no staining.
Accessible and high quality.
Antibody has great signal, highly recommended