Biolegend
Brilliant Violet 650™ anti-mouse CD185 (CXCR5) Antibody
145517
L138D7
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining TFH cells that are CXCR5+PD-1+.
Flow Cytometry
Spleen of C57BL/6
Surface staining with CXCR5 BV650 (1/300), 20 min at 4C
Fc block (2.4G2 clone)
N/A
Flow cytometer
We could stain Tfh cells (CXCR5+PD-1+)
It works well.
It could be brighter.
Can be used, but it requires you to be careful.