Reasonable Anti-mouse PD-1 Antibody

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC/Cyanine7 anti-mouse CD279 (PD-1) Antibody

Catalog Number:

135224

Clone Number:

29F.1A12

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining TFH T cells that are CD4+CXCR5+PD-1+.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

WT naive mouse (spleen)

Primary Incubation

surface staining with anti-PD-1 APC-Cy7 (1/200), 20 min at 4C

Blocking Agent

Fc block (2.4G2 clone)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

We can stain TFH T cells that are CD4+CXCR5+PD-1+.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable

The Bad

Could be brighter

The Bottom Line

It can be used

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