The Good
Packaging, clear protocol,
The Bad
Hard to produce consistent results
The Bottom Line
ELISpots are a variable assay, so it cannot be determined if the kit was at fault for the differences we witnessed. It is best to use whole PBMC's to preserve cell viability without doing any isolations before with this kit. Other kits from R&D have performed well in the past. Optimize with diligence and there should not be an issue.