CRG-2 ELISA Works Great

March 17, 2021

Imanis Life Sciences
218 10th street NW
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Ray Biotech

Product Name:

Mouse CRG-2 ELISA kit

Catalog Number:

ELM-CRG2-1

This kit successfully quantified CRG-2/CXCL10 chemokine in both cell culture supernatants as well as mouse serum in just about 5 hours.

Experimental Design and Results Summary

Application

Detection of CRG-2/CXCL10 chemokine

Starting Material

Cell culture supernatant, serum

Tips

Let standard resuspend for at least 5 minutes.

Results Summary

For cell culture supernatants, neat, 1:10 and 1:100 were adequate loading conditions. For serum, initial dilution of 1:10 is a safe starting point. I incubated my sample for 2.5 hours and this worked well for me.

DOI or PMID #

N/A

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Summary

The Good

Pre-coated plate, one-step substrate solution, extra assay reagents

The Bad

2.5 hour sample incubation, protocol requires gentle shaking

The Bottom Line

This kit works well for quantifying mouse CRG-2/CXCL10 chemokine in cell culture supernatants and serum. I continue to purchase from ray biotech as well as they have great customer service. .

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