Great Gzmb+ Staining

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Fisher Scientific

Product Name:

Granzyme B Mouse anti-Human, PE-Texas Red™, Clone: GB11, Invitrogen

Catalog Number:

GRB17

Clone Number:

GB11

We wanted to examine cytotoxic T cells in the healthy vs diseased state, so we used this antibody to identify Gzmb+ T cells in the spleen.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen cells

Primary Incubation

1:50

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

Attune NxT cytometer

Results Summary

The antibody cross reacts with mouse Gzmb and stains Gzmb+ cells very well in the spleen

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody stains Gzmb+ T cells clearly

The Bad

None to mention

The Bottom Line

The antibody is great for identifying cytotoxic CD8+ T cells and NK cells

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