Antibody Works Great for Staining CD19+ Cell

April 22, 2021

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Fisher Scientific

Product Name:

CD19 Mouse anti-Human, Super Bright 702, Clone: HIB19, eBioscience™

Catalog Number:

67019942

Clone Number:

HIB19

We wanted to examine B cells in the healthy vs diseased state, so we used this antibody to identify B cells in the spleen.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

Attune NxT cytometer

Results Summary

The antibody identifies B cells in the spleen very clearly

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody stains CD19+ B cells very clearly

The Bad

None to mention

The Bottom Line

The antibody is great for identifying B cells in the spleen.

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