Stains NKp46+ Cells Well

May 25, 2021

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 421™ anti-mouse CD335 (NKp46) Antibody

Catalog Number:

137612

Clone Number:

29A1.4

We wanted to examine NK cells in the healthy vs diseased state, so we used this antibody to identify NKp46+ NK cells in the spleen.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen cells

Primary Incubation

1:25

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

Attune NxT cytometer

Results Summary

The antibody can be used to pull out the NKp46+ NK cell population pretty clearly, but the antibody does need to be used at high concentrations

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

The antibody stains NKp46+ NK cells pretty well

The Bad

The antibody needs to be used at high concentrations for a good stain

The Bottom Line

The antibody works for identifying NK cells in the spleen, but needs to be used at a higher concentration.

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