Stains CD317+ Cells

April 22, 2021

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

APC anti-mouse CD317 (BST2, PDCA-1) Antibody

Catalog Number:

127016

Clone Number:

927

We wanted to examine pDCs in the healthy vs diseased state, so we used this antibody to identify CD317+ pDCs in the spleen.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

Attune NxT cytometer

Results Summary

The antibody stains CD317+ cells in the spleen clearly

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody is great for identifying CD317+ cells

The Bad

None to mention

The Bottom Line

The antibody stains CD317+ pDCs clearly

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