Antibody Stains Thy1.2+ Great

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Alexa Fluor® 700 anti-mouse CD90.2 (Thy-1.2) Antibody

Catalog Number:

140324

Clone Number:

53-2.1

We wanted to examine T cells in the healthy vs diseased state, so we used this antibody to identify T cells in the blood.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

single-cell suspension of mouse blood cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

Attune NxT cytometer

Results Summary

The T cells in the blood were very clearly stained with the anti-Thy1.2 antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody identifies Thy1.2+ very well

The Bad

None to mention

The Bottom Line

The antibody is great for pulling out T cells in tissues.

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