Reliable Antibody to Identify CD44 Expression

February 26, 2021

Immunobiology
Yale University
Postdoctoral Researcher

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Company:

BD Biosciences

Product Name:

Rat Anti-Mouse CD44-BV480

Catalog Number:

566116

Clone Number:

IM7

Info: Supplier Page 

Browse Similar Products: CD44 Flow Cytometry Antibodies

CD44 is antigen experienced marker on T cells. Positive expression indicates activated T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine splenocytes

Primary Incubation

1:500

Blocking Agent

FC Block in FACS buffer during surface staining

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Cytek Aurora (3 laser, BVR)

Results Summary

We can identify CD44+ T cells from naive (CD44-) in the spleen.

DOI or PMID #

NA

Additional Notes

Titrate antibody based on tissue samples

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Summary

The Good

Robustly identifies CD44+ T cells in the tumor.

The Bad

None

The Bottom Line

Great reliable antibody to identify CD44+ T cells.

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