Great Flow Staining for KLRG1

February 26, 2021

Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

PE-CF594 Hamster Anti-Mouse KLRG1

Catalog Number:

565393

Clone Number:

N/A

Info: Supplier Page 

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In order to gate out CD8s that are KLRG1+CD127- effector T cells, this antibody works well for us to look at memory versus effector cell types in our experiments.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine T cells

Primary Incubation

1:1000 in FACS Buffer

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Celesta

Results Summary

This antibody works very well for when we want to look at short-lived effector cells (KLRG+CD127-). Our flow graphs always show distinct populations of KLRG- and KLRG+ cells which helps divide out the population of memory precursors and short lived effector cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Antibody works well every time and clear positive and negative

The Bad

There is sometimes spilling over with our antibody, might just be the age of the product though

The Bottom Line

This antibody works well across multiple batches now and it definitely helps gate out KLRG1+ and KLRG1- well.

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