Great Way to Look at CD27 Expression on CD8s

Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 510™ anti-mouse/rat/human CD27

Catalog Number:

124229

Clone Number:

LG.3A10

Our lab is interested in murine CD8s and CD4s therefore using this antibody to look at lymphocytes specifically expressing high or low levels of CD27s. This is one of the molecules used to look at effectors versus memory T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine T cells

Primary Incubation

1:500 in FACS buffer

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow Celesta

Results Summary

This antibody works very well when we want to see if you are looking at CD4 cells expressing CD27. Since stimulation of this molecule is needed for persistence and survival after initial activation, this molecule is interesting to look into.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very good signal

The Bad

None

The Bottom Line

This antibody works very well but definitely need to titrate concentration.

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