Best for Genotyping

February 26, 2021

Oncology
Johns Hopkins School of Medicine
Lab Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

Alexa Fluor® 700 anti-rat CD90/mouse CD90.1 (Thy-1.1) Antibody

Catalog Number:

202528

Clone Number:

OX-7

For our lab, we genotype most of our colony of mice. When we have multiple transgenic lines like P14, OTI, OTII, etc it is extremely useful to also have Thy1.1/1.2 markers to distinguish when we co-adoptive transfer cells into a different Wild type host expressing Thy1.2.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine T Cells

Primary Incubation

1:2000 in FACS buffer

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow

Results Summary

Like any flow antibody, there has to be a titration depending on the detection of your machine. For us, 1:2000 works perfectly without being too bright or dim. We have consistently used this for genotyping our mice.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Very consistent quality every batch we get

The Bad

Like any flow antibody the price is always steep but worth the investment

The Bottom Line

If you want a reliable antibody that has been consistent great for the clone, this antibody is a must buy.

Share your experience with other scientists. Write a Review! »

Join the discussion