Santa Cruz Biotechnology
alpha-tubulin
sc-8035
Info: Supplier Page View Product Specs View Product
Browse Similar Products: alpha Tubulin Western Blot Antibodies
Protein expression in Hela S3 has been tested and normalized against the tubulin. The signal obtained after incubation with the following antibody is very strong and specific.
Western Blot
Hela cells, WCL
1:1000 milk 5% in PBS 0,05% tween
Milk 5% in PBS 0,05% tween
Mouse 1:3000 milk 1% in PBS 0,05% tween
none
ECL
This antibody is very clean and without unspecific bands. Hela cells WCL has been loaded on a 4-12% SDS-precast gel (1:15h; 180 V) then proteins have been transferred on nitrocellulose membrane using the semi-dry blotting (1h 11V). Blocking with milk 5% in PBS 0,05% tween (1 hour) was followed by incubation 1h at RT with GAPDH antibody (1:3000 in milk 5% in PBS 0,05% tween) Then the membrane was washed with 3x 5 minutes with PBS 0,05% tween, followed by a 1-hour incubation with Mouse secondary antibody 1:3000 in milk 1% in PBS 0,05% tween.To wash out the secondary antibody, a second wash with PBS 0,05% tween (3x 5 minutes) was performed. ECL substrate has been used to detect the signal.
The signal is very clean and clear
None
highly recommended