Southern Biotech
Goat Anti-Human IgG(H+L), Mouse/Bovine/Horse SP ads-HRP
2016-05
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It's a secondary antibody conjugated with HRP. We used this antibody for detecting specific human IgGs using the ELISA method. It's a quality antibody, worth the price.
ELISA
Human serum samples
1:3000 dilution in 3% BSA in PBS. 45 minutes at room temperature.
3% BSA in PBS
None
TMB solution used as substrate. OD at 450 nm after stopping the reaction.
We commonly use this secondary antibody for human serum samples. We found the best results at 1:3000 dilution. With this, TMB solution works really well.
Price and quality.
N/A
Quality secondary antibody for ELISA applications. Recommended.