DSHB
Mouse anti-Nkx2.2 antibody
74.5A5
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Browse Similar Products: Anti-NKX2-2 Immunofluorescence Antibodies
Decent antibody to label Nkx2.2 positive cells. The staining tends to be relatively weak so antigene retrieval is necessary. For the price, it is a good antibody.
Immunofluorescence
Mouse embryo E11.5: Spinal cord
1:50, Overnight incubation at 4C in 2%Normal donkey serum in 0,3% triton-x100 in PBS
2%Normal donkey serum in 0,3% triton-x100 in PBS
2h incubation at RT in 2%Normal donkey serum in 0,3% triton-x100 in PBS
None.
Confocal Microscope
Staining of interneurons and p3 domain. To obtain a good staining it is necessary to do an antigene retrieval. In this case, we boil the samples in the microwave during 5 minutes (with small breaks in between).
Price.
Optimization step.
Overall, for the price this antibody is a good choice.