Sigma
Anti-Rabbit IgG (whole molecule)–Peroxidase antibody produced in goat
A6154
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IgGs are glycoprotein antibodies responsible for several immune responses. Rabbit IgGs against target proteins are often used as primary antibodies in various research applications such as western blot. Thus, secondary anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the analysis of target proteins. Goat Anti-Rabbit IgG (whole molecule)-Peroxidase antibody is immunospecific for rabbit IgG by immunoelectrophoresis against normal serum and rabbit IgG, prior to conjugation.
Western Blot
Mouse lysate
1:1000 dilution prepared in 1x TBST
5% milk with 1x TBST
1:4000 dilution in 5% milk prepared in 1xTBST
None
ECL
We commonly used this secondary antibody for rabbit primary antibody to detect our specific band of interest.
This secondary antibody is very good for western blot experiment
It works great for western blot. Highly recommended.