FITC Annexin V to Detect Apoptotic Cells

September 18, 2020

University of Rochester Medical Center
Microbiology & Immunology
Research Scientist

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Company:

Biolegend

Product Name:

FITC Annexin V

Catalog Number:

640905

Annexin V staining is the most reliable tool to detect cells undergoing apoptosis. Biolegend provides Annexin V protein with various fluorescent tags so that it is very useful to analyze cell apoptosis together with other dyes or antibodies. FITC annexin V is compatible with 7-AAD, a cell impermeable nucleic acid dye that is frequently used together in flow cytometry and FITC conjugation is the least inexpensive option.

Experimental Design and Results Summary

Application

Detection of apoptotic cells usually by flow cytometry

Starting Material

N/A

Tips

For Annexing V staining, home-made buffer (10-20mM HEPES saline with 1-3mM CaCl2) works as well as any commercially available buffer

Results Summary

Attached assays are showing the progression of apoptosis in mouse neutrophils. Bone marrow-derived neutrophils (100,000 cells) were incubated with FasL for various periods of time. The cells were then stained with 2.5 ul of the reagent in 100 ul buffer for 15 min at 4 degree.

DOI or PMID #

N/A

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Summary

The Good

Reliable and inexpensive staining of apoptotic cells

The Bad

None

The Bottom Line

FITC Annexin V reliably stains apoptotic cells. FITC fluorophore is compatible with many other fluorophores in flow cytometry, so this is also very useful to detect apoptotic cells from tissues.

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